EMDB EMD-8539

Single particle reconstruction
4.6Å resolution

Cryo-EM map of protofilament of microtubule doublet

Map released:
Last modified:
Overview of EMD-8539
Source organism: Tetrahymena thermophila [5911]
Fitted atomic model: 5ucy
Related EM entries by publication: EMD-8528, EMD-8532, EMD-8537
Primary publication:
Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins.
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun 8 15035-15035 (2017)
PMID: 28462916

Function and Biology Details

Sample name: Protofilament from ciliary microtubule doublet
Cellular component: Protofilament from ciliary microtubule doublet
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE
Proteins: Tubulin alpha chain, Tubulin beta chain

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: AV3
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)