EMDB EMD-8519

Single particle reconstruction
4.9Å resolution

Structure of Eukaryotic CMG Helicase at a Replication Fork and Implications

Map released:
Last modified:
Overview of EMD-8519
Fitted atomic model: 5u8t
Related EM entry by publication: EMD-8518
Primary publication:
Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation.
Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O'Donnell ME
Proc. Natl. Acad. Sci. U.S.A. 114 E697-E706 (2017)
PMID: 28096349

Function and Biology Details

Sample name: CMG-ssDNA
Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Nucleic acid: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
Proteins: CMG-ssDNA, DNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, Minichromosome maintenance protein 5, DNA replication licensing factor MCM6, DNA replication licensing factor MCM7, DNA replication complex GINS protein PSF1, DNA replication complex GINS protein PSF2, DNA replication complex GINS protein PSF3, DNA replication complex GINS protein SLD5, Cell division control protein 45

Experimental Information Details

Resolution: 4.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)