EMDB EMD-8483

Single particle reconstruction
4.6Å resolution

Structure of higher-order HIV-1 strand transfer complex intasome

Map released:
Last modified:
Overview of EMD-8483
Source organisms:
Related EM entry by publication: EMD-8481
Primary publication:
Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome.
Passos DO, Li M, Yang R, Rebensburg SV, Ghirlando R, Jeon Y, Shkriabai N, Kvaratskhelia M, Craigie R, Lyumkis D
Science 355 89-92 (2017)
PMID: 28059769

Function and Biology Details

Sample name: complex formed by a higher-order assembly of Sso7d-fusion HIV-1 Integrase with with IN-binding domain of LEDGF/p75, and the product of DNA strand transfer
Nucleic acids: DNA (11-MER), DNA (23-MER), DNA (37-MER)
Proteins: complex formed by a higher-order assembly of Sso7d-fusion HIV-1 Integrase with with IN-binding domain of LEDGF/p75, and the product of DNA strand transfer, HIV-1 Integrase, Sso7d chimera, Integrase binding domain of LEDGF/p75

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)