EMDB EMD-8469

Single particle reconstruction
4.2Å resolution

3D cryo-EM reconstruction of nucleotide-free MsbA in lipid nanodisc

Map released:
Last modified:
Overview of EMD-8469
Source organisms:
Fitted atomic model: 5tv4
Related EM entries by publication: EMD-8465, EMD-8467, EMD-8669, EMD-8670, EMD-8671
Primary publication:
Structural basis of MsbA-mediated lipopolysaccharide transport.
Mi W, Li Y, Yoon SH, Ernst RK, Walz T, Liao M
Nature 549 233-237 (2017)
PMID: 28869968

Function and Biology Details

Sample name: MsbA reconstituted in lipid nanodisc
Ligands: PHOSPHATE ION, 3-HYDROXY-TETRADECANOIC ACID, MYRISTIC ACID, LAURIC ACID
Proteins: MsbA reconstituted in lipid nanodisc, Lipid A export ATP-binding/permease protein MsbA

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)