EMDB EMD-8454

Single particle reconstruction
3.95Å resolution

Cryo-electron microscopy structure of a bovine CLC-K chloride channel, alternate (class 2) conformation

Map released:
Last modified:
Overview of EMD-8454
Source organisms:
Fitted atomic model: 5tr1
Related EM entry by publication: EMD-8435
Primary publication:
Structure of a CLC chloride ion channel by cryo-electron microscopy.
Park E, Campbell EB, MacKinnon R
Nature 541 500-505 (2017)
PMID: 28002411

Function and Biology Details

Sample name: A bovine CLC-K channel complexed with a monoclonal antibody fragment (Fab)
Ligand: CHOLESTEROL HEMISUCCINATE
Proteins: A bovine CLC-K channel complexed with a monoclonal antibody fragment (Fab), Chloride channel protein, Monoclonal antibody, Fab fragment, heavy chain, Monoclonal antibody, Fab fragment, light chain

Experimental Information Details

Resolution: 3.95Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)