EMDB EMD-8138

Single particle reconstruction
3.9Å resolution

Human core-PIC in the initial transcribing state (no IIS)

Map released:
Last modified:
Overview of EMD-8138
Source organism: Human [9606]
Fitted atomic model: 5iyd
Related EM entries by publication: EMD-8131, EMD-8132, EMD-8133, EMD-8134, EMD-8135, EMD-8136, EMD-8137
Primary publication:
Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature 533 359-365 (2016)
PMID: 27193682

Function and Biology Details

Sample name: Human core-PIC in the initial transcribing state (no IIS)
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: SCP-X, SCP-Y, RNA
Proteins: Human core-PIC in the initial transcribing state (no IIS), RNA polymerase II, General transcription factors, Super core promoter, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit RPB5, DNA-directed RNA polymerase II subunit RPB6, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerase II subunit RPB8, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit RPB10, DNA-directed RNA polymerase II subunit RPB11-a, DNA-directed RNA polymerase II subunit RPB12, Transcription initiation factor IIB, Transcription initiation factor IIA subunit 1, Transcription initiation factor IIA subunit 2, TATA-box-binding protein, General transcription factor IIE subunit 1, Transcription initiation factor IIE subunit beta, General transcription factor IIF subunit 1, General transcription factor IIF subunit 2

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)