EMDB EMD-8133

Single particle reconstruction
7.9Å resolution

Human holo-PIC in the initial transcribing state

Map released:
Last modified:
Overview of EMD-8133
Source organism: Human [9606]
Fitted atomic model: 5iy8
Related EM entries by publication: EMD-8131, EMD-8132, EMD-8134, EMD-8135, EMD-8136, EMD-8137, EMD-8138
Primary publication:
Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature 533 359-365 (2016)
PMID: 27193682

Function and Biology Details

Sample name: Human holo-PIC in the initial transcribing state
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: SCP-X, SCP-Y
Proteins: Human holo-PIC in the initial transcribing state, RNA polymerase II, General transcription factors, Super core promoter, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit RPB5, DNA-directed RNA polymerase II subunit RPB6, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerase II subunit RPB8, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit RPB10, DNA-directed RNA polymerase II subunit RPB11-a, DNA-directed RNA polymerase II subunit RPB12, Transcription initiation factor IIB, Transcription initiation factor IIA subunit 1, Transcription initiation factor IIA subunit 2, TATA-box-binding protein, General transcription factor IIE subunit 1, Transcription initiation factor IIE subunit beta, General transcription factor IIF subunit 1, General transcription factor IIF subunit 2, Transcription elongation factor A protein 1, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH basal transcription factor complex helicase XPD subunit, General transcription factor IIH subunit 2, General transcription factor IIH subunit 5, General transcription factor IIH subunit 4, General transcription factor IIH subunit 3

Experimental Information Details

Resolution: 7.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)