EMDB EMD-8131

Single particle reconstruction
10.0Å resolution

Human core TFIIH bound to DNA within the PIC

Map released:
Last modified:
Overview of EMD-8131
Source organism: Human [9606]
Fitted atomic model: 5ivw
Related EM entries by publication: EMD-8132, EMD-8133, EMD-8134, EMD-8135, EMD-8136, EMD-8137, EMD-8138
Primary publication:
Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature 533 359-365 (2016)
PMID: 27193682

Function and Biology Details

Sample name: Human core TFIIH bound to DNA within the PIC
Nucleic acids: scp-X, scp-Y
Proteins: Human core TFIIH bound to DNA within the PIC, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH basal transcription factor complex helicase XPD subunit, General transcription factor IIH subunit 2, General transcription factor IIH subunit 5, General transcription factor IIH subunit 4, General transcription factor IIH subunit 3

Experimental Information Details

Resolution: 10.0Å
Resolution method: FSC 0.5 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)