Single particle reconstruction
3.28Å resolution

Structure of TRPV1 determined in lipid nanodisc

Map released:
Last modified:
Overview of EMD-8118
Source organism: Rattus norvegicus [10116]
Fitted atomic model: 5irz
Related EM entries by publication: EMD-8117, EMD-8119, EMD-8120
Primary publication:
TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action.
Gao Y, Cao E, Julius D, Cheng Y
Nature 534 347-351 (2016)
PMID: 27281200

Function and Biology Details

Sample name: TRPV1 ion channel in unliganded state
Ligands: (4R,7S)-4-hydroxy-N,N,N-trimethyl-4,9-dioxo-7-[(pentanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphatetradecan-1-aminium, (2S)-1-{[(R)-hydroxy{[(1R,2R,3S,4S,5S,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}-3-(pentanoyloxy)propan-2-yl decanoate, (2S)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(hexanoyloxy)propyl hexanoate
Proteins: TRPV1 ion channel in unliganded state, Transient receptor potential cation channel subfamily V member 1

Experimental Information Details

Resolution: 3.28Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C4
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)