EMDB EMD-8004

Single particle reconstruction
3.8Å resolution

RNC in complex with SRP with detached NG domain

Map released:
Last modified:
Overview of EMD-8004
Source organism: Escherichia coli [562]
Fitted atomic model: 5gah
Related EM entries by publication: EMD-8000, EMD-8001, EMD-8002, EMD-8003
Primary publication:
Structures of the E. coli translating ribosome with SRP and its receptor and with the translocon.
Jomaa A, Boehringer D, Leibundgut M, Ban N
Nat Commun 7 10471-10471 (2016)
PMID: 26804923

Function and Biology Details

Sample name: Ribosome nascent chain complex with srp (NG-detached)
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: SRP 4.5S RNA, tRNA CCAend, 23S rRNA, 5S rRNA
Proteins: Ribosome nascent chain complex with srp (NG-detached), 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, Signal recognition particle protein Ffh, 1A9L SS

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)