EMDB EMD-7888

Single particle reconstruction
20.0Å resolution

Negative Stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3418 at post boost 2.

Map released:
Overview of EMD-7888
Source organism: Oryctolagus cuniculus [9986]
Primary publication:
Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.
Bianchi M, Turner HL, Nogal B, Cottrell CA, Oyen D, Pauthner M, Bastidas R, Nedellec R, McCoy LE, Wilson IA, Burton DR, Ward AB, Hangartner L
Immunity 49 288-300.e8 (2018)
PMID: 30097292

Function and Biology Details

Sample name: Negative stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3418 at post boost 2. Serum digested and Fab purified before adding trimer.
Protein: Negative stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from rabbit 3418 at post boost 2. Serum digested and Fab purified before adding trimer.

Experimental Information Details

Resolution: 20.0Å
Resolution method: FSC 0.5 CUT-OFF
Applied symmetry: C1
Microscope: FEI TECNAI SPIRIT
Detector: TVIPS TEMCAM-F416 (4k x 4k)