EMD-7880

Tomography
EMD-7880
Gallery
Deposition: 15/05/2018
Map released: 15/05/2019
Last modified: 27/05/2020
Overview Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-7880
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Infectious microvesicles (Class 1 and 3) from poliovirus-infected HeLa cells, displaying clusters of virions and additional inner vesicular structures

EMD-7880

Tomography
EMD-7880
Gallery
Deposition: 15/05/2018
Map released: 15/05/2019
Last modified: 27/05/2020
Overview Sample Experiment Validation Additional data Links
Method: Tomography
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1.5 mg/mL
Buffer
pH: 7.0
Details: 1% glutaraldehyde in 1x PBS
Grid
Mesh: 200
Material: COPPER
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 283.15 K
Instrument: FEI VITROBOT MARK II
Fiducial Markers
Manufacturer Fiducial type Diameter
Ted Pella Inc. - 10 nanometer
Sectioning
Details: NO SECTIONING
Microscopy [1]
Microscope: JEOL 2200FS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: DIRECT ELECTRON DE-20 (5k x 3k)
Average electron dose per image: 1.02 e/Å2
Image processing [1]
Final reconstruction
Number of images used: 62
Software [1]
Name Version Details
ETomo - -
Tomogram
Format: CCP4
Data type: IMAGE STORED AS SIGNED BYTE
Annotation details: Infectious microvesicles secreted from poliovirus-infected HeLa cells, displaying clusters of virions and additional inner vesicular structures
⬡ Geometry
X Y Z
Origin -886 -2414 442
Dimensions (px) 753 843 431
Dimensions (Å) 3019.5303 3380.4302 1728.31
Voxel size (Å) 4.01 4.01 4.01
Contour list
Primary Level Source
True - AUTHOR