EMDB EMD-7527

Single particle reconstruction
20.0Å resolution

Class 1 vertex with 2-fold symmetry extracted from SEC13/SEC31deltaC cuboctahedrons and aligned, both using localized reconstruction method.

Map released:
Last modified:
Overview of EMD-7527
Source organism: Homo sapiens [9606]
Primary publication:
Flexibility of the Sec13/31 cage is influenced by the Sec31 C-terminal disordered domain.
Paraan M, Bhattacharya N, Uversky VN, Stagg SM
J. Struct. Biol. 204 250-260 (2018)
PMID: 30172710

Function and Biology Details

Sample name: Assembly of 24 SEC13/SEC31deltaC heterotetramers into a cuboctahedron for which localized reconstruction reveals vertices (4 heterotetramers).
Protein: Assembly of 24 SEC13/SEC31deltaC heterotetramers into a cuboctahedron for which localized reconstruction reveals vertices (4 heterotetramers).

Experimental Information Details

Resolution: 20.0Å
Resolution method: FSC 0.5 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: DIRECT ELECTRON DE-20 (5k x 3k)