EMDB EMD-7517

Single particle reconstruction
4.5Å resolution

Rhodopsin-Gi

Map released:
Last modified:
Overview of EMD-7517
Source organisms:
Fitted atomic model: 6cmo
Primary publication:
Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
Kang Y, Kuybeda O, de Waal PW, Mukherjee S, Van Eps N, Dutka P, Zhou XE, Bartesaghi A, Erramilli S, Morizumi T, Gu X, Yin Y, Liu P, Jiang Y, Meng X, Zhao G, Melcher K, Ernst OP, Kossiakoff AA, Subramaniam S, Xu HE
Nature 558 553-558 (2018)
PMID: 29899450

Function and Biology Details

Sample name: Protein complex of Rhodopsin with Galphai
Ligand: N-ACETYL-D-GLUCOSAMINE
Proteins: Protein complex of Rhodopsin with Galphai, chimera protein of Soluble cytochrome b562 and Rhodopsin, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Fab light chain, Fab Heavy chain

Experimental Information Details

Resolution: 4.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)