EMDB EMD-7460

Single particle reconstruction
3.6Å resolution

Cryo-EM structure at 3.6 A resolution of vaccine-elicited antibody vFP16.02 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122

Map released:
Last modified:
Overview of EMD-7460
Source organisms:
Fitted atomic model: 6cdi
Related EM entries by publication: EMD-7459, EMD-8420, EMD-8421, EMD-8422
Primary publication:
Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1.
Xu K, Acharya P, Kong R, Cheng C, Chuang GY, Liu K, Louder MK, O'Dell S, Rawi R, Sastry M, Shen CH, Zhang B, Zhou T, Asokan M, Bailer RT, Chambers M, Chen X, Choi CW, Dandey VP, Doria-Rose NA, Druz A, Eng ET, Farney SK, Foulds KE, Geng H, Georgiev IS, Gorman J, Hill KR, Jafari AJ, Kwon YD, Lai YT, Lemmin T, McKee K, Ohr TY, Ou L, Peng D, Rowshan AP, Sheng Z, Todd JP, Tsybovsky Y, Viox EG, Wang Y, Wei H, Yang Y, Zhou AF, Chen R, Yang L, Scorpio DG, McDermott AB, Shapiro L, Carragher B, Potter CS, Mascola JR, Kwong PD
Nat. Med. 24 857-867 (2018)
PMID: 29867235

Function and Biology Details

Sample name: vFP20.01-BG505 DS-SOSIP-VRC03-PGT122
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: vFP20.01-BG505 DS-SOSIP-VRC03-PGT122, VRC03, PGT122, Glycoprotein, vFP16.02, Glycoprotein gp41, Glycoprotein 120, PGT122 Light Chain, PGT122 Heavy Chain, VRC03 light chain, VRC03 Heavy Chain, vFP16.02 Heavy Chain, vFP16.02 Light Chain

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)