EMD-7441

Single-particle
3.9 Å
EMD-7441
3D View Gallery
Deposition: 29/01/2018
Map released: 07/03/2018
Last modified: 13/03/2024
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7441
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

CryoEM structure of human SLC4A4 sodium-coupled acid-base transporter NBCe1

EMD-7441

Single-particle
3.9 Å
EMD-7441
3D View Gallery
Deposition: 29/01/2018
Map released: 07/03/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.40 mg/mL
Details: Sample was purified in DDM and exchanged into Amphipols (PMAL-C8)
Buffer
pH: 7.5
Buffer components [2]:
Name Formula Concentration ChEBI
Tris-HCl C4H12ClNO3 20.0 mM
Sodium chloride NaCl 150.0 mM
Grid
Mesh: 300
Model: Quantifoil R1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Calibrated magnification: 36764.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Specialist optics
Energy filter
Name: GIF Quantum LS
Lower energy threshold: 0 eV
Upper energy threshold: 20 eV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Frames per image: 2-36
Number of grids: 2
Number of real images: 3378
Average electron dose per image: 24.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 184561
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
RELION 2.0 -
Startup model [1]
Type: OTHER
Details:Used a oval-shaped disk low-passed filtered to 60A
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 2.0 -
Particle selection [1]
Selected Ref. model Method Software Details
1729419 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Electrogenic Sodium Bicarbonate Cotransporter, SLC4A4, NBCe1
⬡ Geometry
X Y Z
Dimensions 192 192 192
Origin 0 0 0
Spacing 192 192 192
Voxel size 1.36 Å 1.36 Å 1.36 Å
Contour list
Primary Level Source
True 0.075 AUTHOR