EMD-73645

Single-particle
2.8 Å
EMD-73645
3D View Gallery
Deposition: 29/10/2025
Map released: 15/07/2026
Last modified: 29/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-73645

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9yyh summary report (pdf.gz) Map-9yyh FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

AI-generated RNA-guided nuclease R-loop formed state

EMD-73645

Single-particle
2.8 Å
EMD-73645
3D View Gallery
Deposition: 29/10/2025
Map released: 15/07/2026
Last modified: 29/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Deinococcus radiodurans
Sample: AI-designed RNA-guided nuclease R-loop formed state
Fitted models: 9yyh

Deposition Authors: Skopintsev P, Esain-Garcia I, Doudna J
Structure and evolution-guided design of minimal RNA-guided nucleases.
Skopintsev P, Esain-Garcia I, DeTurk EC, Yoon PH, Zhou Z, Weiss T, Kamalu M, Chamraj A, Loi KJ, Langeberg CJ, Boger RS, Nisonoff H, Karp HM, Chen LX, Shi H, Vohra K, Banfield JF, Cate JHD, Jacobsen SE, Doudna JA
(2026) Science , 393 , 313 - 318
PUBMED: 42462008
DOI: doi:10.1126/science.aed6123
ISSN: 1095-9203
ASTM: SCIEAS