EMD-71101

Single-particle
4.0 Å
EMD-71101
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Deposition: 10/06/2025
Map released: 22/10/2025
Last modified: 17/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-71101
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EMICSS annotations (xml)

Cas1-2/3 integration complex, 34 bp foreign DNA with PAM, first transesterification

EMD-71101

Single-particle
4.0 Å
EMD-71101
3D View Gallery
Deposition: 10/06/2025
Map released: 22/10/2025
Last modified: 17/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Pseudomonas aeruginosa
Sample: Integration complex formed by Cas1-2/3 integrase with integration host factor alpha and beta, the CRISPR leader and CRISPR array, and a short foreign DNA fragment

Deposition Authors: Henriques WS, Bowman J, Hall LN, Gauvin CG, Wei H, Kuang H, Zimanyi CM, Eng ET, Santiago-Frangos A, Wiedenheft B
Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci.
Henriques WS, Bowman J, Hall LN, Gauvin CC, Wei H, Kuang H, Zimanyi CM, Eng ET, Santiago-Frangos A, Wiedenheft B
(2025) Structure , 33 , 2058 - 2070.e6
PUBMED: 41072406
DOI: doi:10.1016/j.str.2025.09.007
ISSN: 0969-2126
ASTM: STRUE6