EMDB EMD-7050

Single particle reconstruction
3.1Å resolution

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1

Map released:
Overview of EMD-7050
Fitted atomic model: 6b46
Related in-frame EM entries: EMD-7048, EMD-7049, EMD-7051, EMD-7052
Related EM entries by publication: EMD-7048, EMD-7049, EMD-7051, EMD-7052
Primary publication:
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell 171 414-426.e12 (2017)
PMID: 28985564

Function and Biology Details

Sample name: Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1
Nucleic acid: Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence
Proteins: Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1, CRISPR-associated protein Csy3, Anti-CRISPR protein AcrF1, CRISPR-associated endonuclease Cas6/Csy4

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)