EMDB EMD-7025

Single particle reconstruction
2.5Å resolution

Cryo-EM structure of of the large subunit of Leishmania ribosome bound to paromomycin

Map released:
Last modified:
Overview of EMD-7025
Source organism: Leishmania donovani [5661]
Fitted atomic model: 6az3
Related EM entry by publication: EMD-7024
Primary publication:
Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin.
Shalev-Benami M, Zhang Y, Rozenberg H, Nobe Y, Taoka M, Matzov D, Zimmerman E, Bashan A, Isobe T, Jaffe CL, Yonath A, Skiniotis G
Nat Commun 8 1589-1589 (2017)
PMID: 29150609

Function and Biology Details

Sample name: Leishmania donovani 91S ribosome LSU
Ligands: PAROMOMYCIN, MAGNESIUM ION, CYTIDINE-5'-MONOPHOSPHATE, water
Nucleic acids: rRNA alpha, rRNA beta, rRNA gamma, rRNA delta, rRNA epsilon, rRNA zeta, rRNA 5.8S, rRNA 5S
Proteins: Leishmania donovani 91S ribosome LSU, ribosomal protein uL2, ribosomal protein uL3, ribosomal protein uL4, ribosomal protein uL5, ribosomal protein uL6, ribosomal protein eL6, ribosomal protein eL8, ribosomal protein uL13, ribosomal protein eL13, ribosomal protein uL14, ribosomal protein eL14, ribosomal protein uL15, ribosomal protein eL15, ribosomal protein uL16, ribosomal protein uL18, ribosomal protein eL18, ribosomal protein eL19, ribosomal protein eL20, ribosomal protein eL21, ribosomal protein uL22, ribosomal protein eL22, ribosomal protein uL23, ribosomal protein uL24, ribosomal protein eL24, ribosomal protein eL27, ribosomal protein eL28, ribosomal protein uL29, ribosomal protein eL29, ribosomal protein uL30, ribosomal protein eL30, ribosomal protein eL31, ribosomal protein eL32, ribosomal protein eL33, ribosomal protein eL34, ribosomal protein eL36, ribosomal protein eL37, ribosomal protein eL38, ribosomal protein eL39, ribosomal protein eL40, ribosomal protein eL41, ribosomal protein eL43, ribosomal protein eL44

Experimental Information Details

Resolution: 2.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)