EMDB EMD-7010

Single particle reconstruction
3.8Å resolution

Cryo-EM structure of human immunoproteasome with a novel noncompetitive inhibitor that selectively inhibits activated lymphocytes

Map released:
Overview of EMD-7010
Source organism: Homo sapiens [9606]
Fitted atomic model: 6avo
Primary publication:
Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes.
Santos RLA, Bai L, Singh PK, Murakami N, Fan H, Zhan W, Zhu Y, Jiang X, Zhang K, Assker JP, Nathan CF, Li H, Azzi J, Lin G
Nat Commun 8 1692-1692 (2017)
PMID: 29167449

Function and Biology Details

Sample name: human immunoproteasome with a novel noncompetitive inhibitor that selectively inhibits activated lymphocytes
Ligand: N~1~-{2-[([1,1'-biphenyl]-3-carbonyl)amino]ethyl}-N~4~-tert-butyl-N~2~-(3-phenylpropanoyl)-L-aspartamide
Proteins: human immunoproteasome with a novel noncompetitive inhibitor that selectively inhibits activated lymphocytes, Proteasome subunit beta type-9, Proteasome subunit beta type-10, Proteasome subunit beta type-8, Proteasome subunit alpha type-1, Proteasome subunit alpha type-5, Proteasome subunit alpha type-7, Proteasome subunit alpha type-3, Proteasome subunit alpha type-6, Proteasome subunit alpha type-4, Proteasome subunit alpha type-2, Proteasome subunit beta type-1, Proteasome subunit beta type-2, Proteasome subunit beta type-3, Proteasome subunit beta type-4

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: JEOL 3200FS
Detector: GATAN K2 (4k x 4k)