EMDB EMD-6864

Single particle reconstruction
4.1Å resolution

The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution

Map released:
Overview of EMD-6864
Source organism: Human [9606]
Fitted atomic model: 5yzg
Primary publication:
Structure of a human catalytic step I spliceosome
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science 359 537-545 (2018)
PMID: 29301961

Function and Biology Details

Sample name: Human Catalytic Step I Spliceosome
Ligands: INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ZINC ION, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: U5 snRNA, U6 snRNA, Pre-mRNA, U2 snRNA
Proteins: Human Catalytic Step I Spliceosome, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 small nuclear ribonucleoprotein 40 kDa protein, Pre-mRNA-splicing factor SYF1, Crooked neck-like protein 1, Pre-mRNA-splicing factor SPF27, Cell division cycle 5-like protein, Pre-mRNA-splicing factor ISY1 homolog, Pre-mRNA-splicing factor SYF2, Protein BUD31 homolog, Pre-mRNA-splicing factor RBM22, Spliceosome-associated protein CWC15 homolog, SNW domain-containing protein 1, Peptidyl-prolyl cis-trans isomerase-like 1, Pleiotropic regulator 1, Intron-binding protein aquarius, Serine/arginine repetitive matrix protein 2, Pre-mRNA-splicing factor CWC22 homolog, Pre-mRNA-processing factor 17, Pre-mRNA-splicing factor CWC25 homolog, Coiled-coil domain-containing protein 94, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, Pre-mRNA-processing factor 19, Eukaryotic initiation factor 4A-III, Protein mago nashi homolog 2, RNA-binding protein 8A, Protein CASC3, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', Peptidyl-prolyl cis-trans isomerase E, Peptidylprolyl isomerase domain and WD repeat-containing protein 1, Peptidyl-prolyl cis-trans isomerase G, UNKNOW

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)