EMD-67606

Single-particle
2.65 Å
EMD-67606
3D View Gallery
Deposition: 09/12/2025
Map released: 01/07/2026
Last modified: 22/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-67606

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-21ea summary report (pdf.gz) Map-21ea FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of AtCas9-sgRNA-underwound DNA (TATA PAM) ternary complex

EMD-67606

Single-particle
2.65 Å
EMD-67606
3D View Gallery
Deposition: 09/12/2025
Map released: 01/07/2026
Last modified: 22/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Alicyclobacillus tolerans
Sample: AtCas9-sgRNA-underwound DNA (TATA PAM) ternary complex
Fitted models: 21ea

Deposition Authors: Meng B, Duan M, Wu LJ, Liu ZJ, Zhang Y
Structural basis of AtCas9 recognition of PAM mutants in underwound DNA topology.
Duan M, Meng B, Zhou L, Wu L, Tong X, Huang D, Yin H, Liu ZJ, Zhang Y
(2026) Nat Struct Mol Biol , 33 , 1062 - 1074
PUBMED: 42342973
DOI: doi:10.1038/s41594-026-01831-6
ISSN: 1545-9985