EMDB EMD-6747

Single particle reconstruction
3.83Å resolution

RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

Map released:
Last modified:
Overview of EMD-6747
Fitted atomic model: 5xon
Primary publication:
Structure of the complete elongation complex of RNA polymerase II with basal factors
Ehara H, Yokoyama T, Shigematsu H, Yokoyama S, Shirouzu M, Sekine SI
Science 357 921-924 (2017)
PMID: 28775211

Function and Biology Details

Sample name: RNA polymerase II elongation complex bound with Spt4/5 and TFIIS
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: RNA (30-MER), DNA (48-MER), DNA (48-MER)
Proteins: RNA polymerase II elongation complex bound with Spt4/5 and TFIIS, RNA polymerase II, Spt4/5 and TFIIS, DNA-directed RNA polymerase subunit, DNA-directed RNA polymerase subunit beta, RNA polymerase II third largest subunit B44, part of central core, RNA polymerase II subunit B32, RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III, RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III, RNA polymerase II subunit, RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III, DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, RNA polymerase subunit, found in RNA polymerase complexes I, II, and III, General transcription elongation factor TFIIS, Transcription elongation factor SPT4, Protein that forms a complex with Spt4p

Experimental Information Details

Resolution: 3.83Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TECNAI ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)