EMD-6729

Single-particle
3.8 Å
EMD-6729
3D View Gallery
Deposition: 11/05/2017
Map released: 10/01/2018
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-6729
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5xlo summary report (pdf.gz) Map and 5xlo FULL report (pdf.gz)
EMICSS annotations (xml)

Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region

EMD-6729

Single-particle
3.8 Å
EMD-6729
3D View Gallery
Deposition: 11/05/2017
Map released: 10/01/2018
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.4 mg/mL
Buffer
pH: 7.5
Grid
Mesh: 400
Model: Quantifoil R1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Time: 60 s
Atmosphere: AIR
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average exposure time: 0.2 s
Average electron dose per image: 1.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 155099
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2.0 -
Startup model [1]
Type: INSILICO MODEL
Insilico model: Gauss sphere
⦨ Initial angle assignment
Type: RANDOM ASSIGNMENT
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 2.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 180 180 180
Dimensions (Å) 233.99998 233.99998 233.99998
Voxel size (Å) 1.3 1.3 1.3
Contour list
Primary Level Source
True 0.033 AUTHOR