EMDB EMD-6729

Single particle reconstruction
3.8Å resolution

Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region

Map released:
Overview of EMD-6729
Source organism: Pseudomonas aeruginosa (strain UCBPP-PA14) [208963]
Fitted atomic model: 5xlo
Related EM entries by publication: EMD-6728, EMD-6730, EMD-6731
Primary publication:
Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures.
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res. 27 853-864 (2017)
PMID: 28574055

Function and Biology Details

Sample name: anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region
Nucleic acid: crRNA with 32nt spacer sequence
Proteins: anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region, CRISPR-associated protein Csy3, crRNA with 20nt spacer sequence, AcrF1, CRISPR-associated protein Csy3, Uncharacterized protein AcrF1

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)