EMDB EMD-6728

Single particle reconstruction
5.3Å resolution

Anti-CRISPR protein AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA

Map released:
Overview of EMD-6728
Source organism: Pseudomonas aeruginosa [287]
Related EM entries by publication: EMD-6729, EMD-6730, EMD-6731
Primary publication:
Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures.
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res. 27 853-864 (2017)
PMID: 28574055

Function and Biology Details

Sample name: anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA
Nucleic acid: crRNA with 20nt spacer
Proteins: anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA, Csy1, Csy2, Csy3, Csy4, AcrF1, AcrF2

Experimental Information Details

Resolution: 5.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)