EMDB EMD-6710

Single particle reconstruction
3.7Å resolution

Structure of the 30S small subunit of chloroplast ribosome from spinach

Map released:
Last modified:
Overview of EMD-6710
Source organism: Spinach [3562]
Fitted atomic model: 5x8r
Related EM entry by publication: EMD-6709, EMD-6711
Primary publication:
Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation
Ahmed T, Shi J, Bhushan S
Nucleic Acids Res. 45 8581-8595 (2017)
PMID: 28582576

Function and Biology Details

Sample name: 30S small subunit of chloroplast ribosome from spinach
Nucleic acid: 16S rRNA
Proteins: 30S small subunit of chloroplast ribosome from spinach, 30S ribosomal protein S2, chloroplastic, 30S ribosomal protein S3, chloroplastic, 30S ribosomal protein S5, chloroplastic, 30S ribosomal protein S6 alpha, chloroplastic, 30S ribosomal protein S7, chloroplastic, 30S ribosomal protein S8, chloroplastic, 30S ribosomal protein S9, chloroplastic, protein S10, 30S ribosomal protein S11, chloroplastic, 30S ribosomal protein S12, chloroplastic, 30S ribosomal protein S13, chloroplastic, 30S ribosomal protein S15, chloroplastic, 30S ribosomal protein S16, chloroplastic, protein S17, 30S ribosomal protein S18, chloroplastic, 30S ribosomal protein S19 alpha, chloroplastic, protein S20, protein S21, protein plastid pY, protein cS23, 30S ribosomal protein S4, chloroplastic, protein cS22, 30S ribosomal protein S14, chloroplastic, protein bTHXc, 30S ribosomal protein S1, chloroplastic

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)