EMDB EMD-6651

Single particle reconstruction
4.2Å resolution

Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome

Map released:
Overview of EMD-6651
Source organisms:
Primary publication:
Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.
Abeyrathne PD, Koh CS, Grant T, Grigorieff N, Korostelev AA
eLife 5 e14874-e14874 (2016)
PMID: 27159452

Function and Biology Details

Sample name: Saccharomyces cerevisiae 80S ribosome bound to TSV IRES and eEF2 translation initiation complex, Structure III, Map 3
Nucleic acid: Internal Ribosome Entry Site
Protein: eukaryotic elongation factor 2
Ribosome: Ribosome

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C1
Reconstruction software: Unblur, CTFFIND4, FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)