EMD-66348

Single-particle
2.7 Å
EMD-66348
3D View Gallery
Deposition: 25/09/2025
Map released: 24/06/2026
Last modified: 08/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-66348

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9wxj summary report (pdf.gz) Map-9wxj FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a non-cognate target RNA in the pre-cleavage state

EMD-66348

Single-particle
2.7 Å
EMD-66348
3D View Gallery
Deposition: 25/09/2025
Map released: 24/06/2026
Last modified: 08/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Gammaproteobacteria bacterium
Sample: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a non-cognate target RNA in the pre-cleavage state
Fitted models: 9wxj

Deposition Authors: Mitsuda Y, Ishikawa J, Nagahata N, Hiraizumi M, Yamashita K, Nishimasu H
Structural mechanism of SAM-AMP and SAM-AMP 2 synthesis by the type III-D2 CRISPR effector complex.
Mitsuda Y, Sugaya M, Ishikawa J, Nagahata N, Okazaki S, Hiraizumi M, Kato K, Gootenberg JS, Abudayyeh OO, Osawa T, Yamashita K, Nishimasu H
(2026) Nat Commun , 17
PUBMED: 42342668
DOI: doi:10.1038/s41467-026-74422-0
ISSN: 2041-1723