Single particle reconstruction
3.6Å resolution

Glutamate dehydrogenase in complex with NADH and GTP, open conformation

Map released:
Last modified:
Overview of EMD-6632
Source organisms:
Fitted atomic model: 3jd3
Related EM entries by publication: EMD-6630, EMD-6631, EMD-6633, EMD-6634, EMD-6635
Primary publication:
Using cryo-EM to map small ligands on dynamic metabolic enzymes: Studies with glutamate dehydrogenase.
Borgnia MJ, Banerjee S, Merk A, Matthies D, Bartesaghi A, Rao P, Pierson J, Earl LA, Falconieri V, Subramaniam S, Milne JL
MOL.PHARMACOL. 89 645-651 (2016)
PMID: 27036132

Function and Biology Details

Sample name: Ternary complex of bovine glutamate dehydrogenase with GTP and NADH
Ligands: [[(2R,3S,4R,5R)-5-(2-amino-6-oxo-3H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate, [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
Protein: L-glutamate:NAD(P)+ oxidoreductase (deaminating)

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: D3
Reconstruction software: CTFFIND3, EMAN2, Relion
Detector: GATAN K2 SUMMIT (4k x 4k)