EMDB EMD-6631

Single particle reconstruction
3.47Å resolution

Glutamate dehydrogenase in complex with GTP

Map released:
Last modified:
Overview of EMD-6631
Source organisms:
Fitted atomic model: 3jd0
Related EM entries by publication: EMD-6630, EMD-6632, EMD-6633, EMD-6634, EMD-6635
Primary publication:
Using cryo-EM to map small ligands on dynamic metabolic enzymes: Studies with glutamate dehydrogenase.
Borgnia MJ, Banerjee S, Merk A, Matthies D, Bartesaghi A, Rao P, Pierson J, Earl LA, Falconieri V, Subramaniam S, Milne JL
MOL.PHARMACOL. 89 645-651 (2016)
PMID: 27036132

Function and Biology Details

Sample name: Binary complex of bovine glutamate dehydrogenase with GTP
Ligand: [[(2R,3S,4R,5R)-5-(2-amino-6-oxo-3H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
Protein: L-glutamate:NAD(P)+ oxidoreductase (deaminating)

Experimental Information Details

Resolution: 3.47Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: D3
Reconstruction software: CTFFIND3, EMAN2, Relion
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)