EMD-65733

Single-particle
3.83 Å
EMD-65733
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 06/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-65733

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9w7u summary report (pdf.gz) Map-9w7u FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class C

EMD-65733

Single-particle
3.83 Å
EMD-65733
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 06/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes, Streptococcus pyogenes serotype M1
Sample: SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class C
Fitted models: 9w7u

Deposition Authors: Zheng R, Ma LJ
Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions.
Zheng R, Lu Z , Wei R, Shin YC , Du J, Zhang Q, Li J, Wang X, Wei Y, Liu B, Chen Y, Ding L, Zhang H, Chen H, Huang J , Ma L
(2026) Nat Struct Mol Biol , 33 , 590 - 602
PUBMED: 41851507
DOI: doi:10.1038/s41594-026-01753-3
ISSN: 1545-9985
Grant Support: