EMDB EMD-6570

Single particle reconstruction
3.77Å resolution

The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U5 Snu114 region at 3.77 A)

Map released:
Overview of EMD-6570
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 3jcm
Primary publication:
The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis
Wan R, Yan C, Bai R, Wang L, Huang M, Wong CCL, Shi Y
SCIENCE 351 466-475 (2016)
PMID: 26743623

Function and Biology Details

Sample name: U4/U6.U5 tri-snRNP from Saccharomyces cerevisiae
Cellular component: U4/U6.U5 tri-snRNP

Experimental Information Details

Resolution: 3.77Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)