EMDB EMD-6480

Single particle reconstruction
3.8Å resolution

Atomic structure of the Apaf-1 apoptosome

Map released:
Overview of EMD-6480
Source organisms:
Fitted atomic model: 3jbt
Related in-frame EM entry: EMD-6481
Related EM entry by publication: EMD-6481
Primary publication:
Atomic structure of the apoptosome: mechanism of cytochrome c- and dATP-mediated activation of Apaf-1
Zhou MY, Li YN, Hu Q, Bai XC, Huang WY, Yan CY, Scheres SHW, Shi YG
GENES DEV. (2015)
PMID: 26543158

Function and Biology Details

Sample name: Heptameric apoptosome of activated human Apaf-1 binding to horse cytochrome c and dATP
Proteins: The apoptotic protease activating factor 1, cytochrome c

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C7
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)