EMD-63768

Single-particle
2.88 Å
EMD-63768
3D View Gallery
Deposition: 15/03/2025
Map released: 18/03/2026
Last modified: 29/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-63768
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9mb7 summary report (pdf.gz) Map and 9mb7 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex

EMD-63768

Single-particle
2.88 Å
EMD-63768
3D View Gallery
Deposition: 15/03/2025
Map released: 18/03/2026
Last modified: 29/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Staphylococcus aureus
Sample: eSaCas9-NNG-sgRNA-mismatched target DNA ternary complex
Fitted models: 9mb7

Deposition Authors: Omura SN , Nakagawa R , Yamashita K , Nishimasu H , Nureki O
Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation.
Omura SN , Nakagawa R , Kajimoto S, Okazaki S, Ishiguro S, Mori H, Onishi K, Kashiwakura Y, Hiramoto T, Horinaka K, Tanaka M, Hirano H, Jividen K, Yamashita K , Tsai SQ, Yachie N, Ohmori T, Nishimasu H , Nureki O
(2026) Nat Commun , 17
PUBMED: 41991526
DOI: doi:10.1038/s41467-026-71626-2
ISSN: 2041-1723