EMDB EMD-6354

Single particle reconstruction
3.7Å resolution

Asymmetric (C1) reconstruction of EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP and GDP)

Map released:
Last modified:
Overview of EMD-6354
Source organisms:
Fitted atomic model: 3jaw
Primary publication:
Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins
Zhang R, Alushin GM, Brown A, Nogales E
CELL(CAMBRIDGE,MASS.) 162 849-859 (2015)
PMID: 26234155

Function and Biology Details

Sample name: EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP)
Proteins: Alpha tubulin, Beta tubulin, EB3

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C1
Reconstruction software: EMAN1, FREALIGN
Microscope: FEI TITAN
Detector: GATAN K2 (4k x 4k)