EMDB EMD-6319

Subtomogram averaging
11.3Å resolution

Structure of bacterial chemotaxis signaling CheA2-trimer core complex by cryo-electron tomography and subvolume averaging

Map released:
Overview of EMD-6319
Source organism: Escherichia coli [562]
Fitted atomic model: 3ja6
Related EM entry by publication: EMD-3234, EMD-6320
Primary publication:
CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling.
Cassidy CK, Himes BA, Alvarez FJ, Ma J, Zhou G, Perilla JR, Schulten K, Zhang P
eLife 4 e08419-e08419 (2015)
PMID: 26583751

Function and Biology Details

Sample name: tarCF CheA CheW
Proteins: Chemotaxis protein CheA, Chemotaxis protein CheW, Methyl-accepting chemotaxis protein II

Experimental Information Details

Resolution: 11.3Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C3
Reconstruction software: IMOD
Microscope: FEI POLARA 300
Detector: GATAN ULTRASCAN 4000 (4k x 4k)