EMDB EMD-5662

Single particle reconstruction
12.5Å resolution

holo-ACP4-PikAIII/C209A/delta ACP5 (no pentaketide)

Map released:
Last modified:
Overview of EMD-5662
Source organism: Streptomyces venezuelae [54571]
Related EM entries by publication: EMD-5647, EMD-5648, EMD-5649, EMD-5651, EMD-5653
Primary publication:
Structure of a modular polyketide synthase
Dutta S, Whicher JR, Hansen DA, Hale WA, Chemler JA, Congdon GR, Narayan ARH, Hakansson K, Sherman DH, Smith JL, Skiniotis G
NATURE 510 512-517 (2014)
PMID: 24965652

Function and Biology Details

Sample name: The 5th module from the pikromycin biosynthetic pathway (PikAIII) lacking the ACP domain with an N-terminal fusion to the ACP domain from module 4 of the pikromycin pathway (ACP4) - the ACP4 domain was phosphopantetheinylated
Protein: PikAIII

Experimental Information Details

Resolution: 12.5Å
Resolution method: FSC 0.5
Applied symmetry: C2
Reconstruction software: EMAN1, EMAN2
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)