EMDB › EMD-5625

Single particle reconstruction
14Å resolution

Architecture of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 on DNA reveals similarity to DNA polymerase clamp loading complexes

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Overview of EMD-5625
Source organism: Saccharomyces cerevisiae [4932]
Primary publication:
Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA.
Sun J, Evrin C, Samel SA, Fernandez-Cid A, Riera A, Kawakami H, Stillman B, Speck C, Li H
NAT.STRUCT.MOL.BIOL. 20 944-951 (2013)
PMID: 23851460

Function and Biology Details

Sample name: ORC-Cdc6-Cdt1-Mcm2-7 complex
Protein: ORC-Cdc6-Cdt1-Mcm2-7 complex

Experimental Information Details

Resolution: 14Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN1, EMAN2
Microscope: JEOL 2010F
Detector: GATAN ULTRASCAN 4000 (4k x 4k)
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