EMD-5381

Single-particle
15.0 Å
EMD-5381
3D View Gallery
Deposition: 08/01/2012
Map released: 14/03/2012
Last modified: 04/06/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-5381
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cdc6-induced Conformational Changes in ORC Bound To Origin DNA Revealed by Cryo-Electron Microscopy. This map may be mirrored (based on comparison to EMD-5625).

EMD-5381

Single-particle
15.0 Å
EMD-5381
3D View Gallery
Deposition: 08/01/2012
Map released: 14/03/2012
Last modified: 04/06/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.4 mg/mL
Buffer
pH: 7.5
Details: 50 mM HEPES/KOH, pH 7.5, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 100 mM KGlu
Grid
Details: 300 mesh lacey grid with thin continuous carbon
Vitrification
Cryogen name: ETHANE
Chamber humidity: 70%
Instrument: OTHER
Method: Blot 5 seconds
Details: Vitrification instrument: FEI Vitrobot
Microscopy [1]
Microscope: JEOL 2010F
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 3.0 µm - 8.0 µm
Nominal magnification: 60000.0
Calibrated magnification: 60000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626200
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON COOLSCAN
Sampling interval: 6.35 µm
Average electron dose per image: 15 e/Å2
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 15.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 54000
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN1.8 - -
CTF correction
Details: Each particle set
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: CryoEM structure of Yeast Origin Recognition complex with dsDNA and Cdc6. This map may be mirrored (based on comparison to EMD-5625).
Details: ::::EMDATABANK.org::::EMD-5381::::
⬡ Geometry
X Y Z
Origin -24 -24 -24
Dimensions (px) 48 48 48
Dimensions (Å) 203.04001 203.04001 203.04001
Voxel size (Å) 4.23 4.23 4.23
Contour list
Primary Level Source
True 1.0 AUTHOR