Sample Organism: Homo sapiens
Sample: Recombinant human CETP (about 53 kDa before post-translational modifications) was expressed in the dihydrofolate reductase-deficient Chinese hamster ovary cell line DG44. The spherical HDL were converted from discoidal reconstituted HDL (rHDL)by incubation with fatty acid-free bovine serum albumin, beta-mercaptoethanol, ultracentrifugally isolated LDL and purified lecithin-cholesterol acyltransferase (LCAT), and then isolated by sequential ultracentrifugation in the density range of 1.07-1.21 g/ml.

Deposition Authors: Zhang L, Yan F, Zhang S, Lei D, Charles MA, Cavigiolio G, Oda M, Krauss RM, Weisgraber KH, Rye KA, Pownall HJ , Qiu X, Ren G

Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein.