EMD-5347
3D map of CETP-HDL complex at 14 Angstrom by optimized negative-staining EM and single-particle reconstruction
EMD-5347
Single-particle14.0 Å
Deposition: 05/10/2011Map released: 21/05/2012
Last modified: 30/05/2012
Sample Organism:
Homo sapiens
Sample: Recombinant human CETP (about 53 kDa before post-translational modifications) was expressed in the dihydrofolate reductase-deficient Chinese hamster ovary cell line DG44. The spherical HDL were converted from discoidal reconstituted HDL (rHDL)by incubation with fatty acid-free bovine serum albumin, beta-mercaptoethanol, ultracentrifugally isolated LDL and purified lecithin-cholesterol acyltransferase (LCAT), and then isolated by sequential ultracentrifugation in the density range of 1.07-1.21 g/ml.
Deposition Authors: Zhang L, Yan F, Zhang S, Lei D
,
Charles MA,
Cavigiolio G,
Oda M,
Krauss RM,
Weisgraber KH,
Rye KA,
Pownall HJ ,
Qiu X,
Ren G
Sample: Recombinant human CETP (about 53 kDa before post-translational modifications) was expressed in the dihydrofolate reductase-deficient Chinese hamster ovary cell line DG44. The spherical HDL were converted from discoidal reconstituted HDL (rHDL)by incubation with fatty acid-free bovine serum albumin, beta-mercaptoethanol, ultracentrifugally isolated LDL and purified lecithin-cholesterol acyltransferase (LCAT), and then isolated by sequential ultracentrifugation in the density range of 1.07-1.21 g/ml.
Deposition Authors: Zhang L, Yan F, Zhang S, Lei D
,
Charles MA,
Cavigiolio G,
Oda M,
Krauss RM,
Weisgraber KH,
Rye KA,
Pownall HJ ,
Qiu X,
Ren G
Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein.
Zhang L,
Yan F,
Zhang S,
Lei D ,
Charles MA,
Cavigiolio G,
Oda M,
Krauss RM,
Weisgraber KH,
Rye KA,
Pownall HJ ,
Qiu X,
Ren G
(2012) Nat. Chem. Biol. , 8 , 342 - 349
,
Charles MA,
Cavigiolio G,
Oda M,
Krauss RM,
Weisgraber KH,
Rye KA,
Pownall HJ ,
Qiu X,
Ren G
(2012) Nat. Chem. Biol. , 8 , 342 - 349