EMD-52921

Single-particle
5.4 Å
EMD-52921
3D View Gallery
Deposition: 26/02/2025
Map released: 14/05/2025
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-52921

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

RNA polymerase-sigma54-DNA focussed map of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -10 to -1 - open clamp

EMD-52921

Single-particle
5.4 Å
EMD-52921
3D View Gallery
Deposition: 26/02/2025
Map released: 14/05/2025
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli K-12
Sample: Subtracted refinement map of conformation 1 of PspF(1-275)-sigma54 bound to nifH DNA (-28 to +35) containing mismatch from -11 to -8.

Deposition Authors: Gao F , Zhang X
Subunit specialization in AAA+ proteins and substrate unfolding during transcription complex remodeling.
Gao F , Ye F , Buck M , Zhang X
(2025) PNAS , 122 , e2425868122 - e2425868122
PUBMED: 40273105
DOI: doi:10.1073/pnas.2425868122
ISSN: 1091-6490
ASTM: PNASA6