EMDB EMD-5036

Single particle reconstruction
6.7Å resolution

Aminoacyl-tRNA-EF-Tu-GDP-kir ternary complex-bound E. coli 70S ribosome

Map released:
Last modified:
Overview of EMD-5036
Source organism: Escherichia coli [562]
Fitted atomic model: 4v69
Primary publication:
Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis.
Villa E, Sengupta J, Trabuco LG, LeBarron J, Baxter WT, Shaikh TR, Grassucci RA, Nissen P, Ehrenberg M, Schulten K, Frank J
PROC.NAT.ACAD.SCI.USA 106 1063-1068 (2009)
PMID: 19122150

Function and Biology Details

Sample name: 70S ribosome from E. coli complex 70S-fMet-tRNA-Phe-tRNA-EF-Tu-GDP-kirromycin.
Nucleic acids: fMet-tRNAfMet, Phe-tRNAPhe, tRNA
Protein: Elongation factor Tu
Ribosome: 70S ribosome

Experimental Information Details

Resolution: 6.7Å
Resolution method: FCS at 0.5 cut-off criterion, extrapolated to full dataset
Applied symmetry: C1
Reconstruction software: Spider
Microscope: FEI POLARA 300
Detector: KODAK SO-163 FILM