EMD-49594
Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution
EMD-49594
Single-particle2.13 Å
Deposition: 08/03/2025Map released: 18/06/2025
Last modified: 12/08/2026
Sample Organism:
Thermus thermophilus HB8
Sample: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
Fitted models: 9no7
Deposition Authors: Aleksandrova EV
,
Syroegin EA ,
Basu RS ,
Vassilevski AA ,
Gagnon MG ,
Polikanov YS
Sample: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
Fitted models: 9no7
Deposition Authors: Aleksandrova EV
,
Syroegin EA ,
Basu RS ,
Vassilevski AA ,
Gagnon MG ,
Polikanov YS
Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome.
Aleksandrova EV ,
Syroegin EA ,
Basu RS ,
Vassilevski AA ,
Gagnon MG ,
Polikanov YS
(2025) Science , 388 , eads9030 - eads9030
,
Syroegin EA ,
Basu RS ,
Vassilevski AA ,
Gagnon MG ,
Polikanov YS
(2025) Science , 388 , eads9030 - eads9030
Grant Support:
- National Science Foundation (NSF, United States): MCB-1907273 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R01-GM132302 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R35-GM151957 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R01-GM136936 (United States)
- Other government: University of Illinois Startup Funds
- Welch Foundation: H-2032-20230405 (United States)