EMDB EMD-4943

Subtomogram averaging
25.6Å resolution

In situ structure of a pentameric IgM complex bound to complement components C1 and one molecule of C4b

Map released:
Last modified:
Overview of EMD-4943
Source organism: Homo sapiens [9606]
Related EM entry by publication: EMD-4878, EMD-4945
Primary publication:
Insights into IgM-mediated complement activation based on in situ structures of IgM-C1-C4b.
Sharp TH, Boyle AL, Diebolder CA, Kros A, Koster AJ, Gros P
Proc.Natl.Acad.Sci.USA 116 11900-11905 (2019)
PMID: 31147461

Function and Biology Details

Sample name: pentameric IgM-C1-C4b1
Protein: pentameric IgM-C1-C4b1

Experimental Information Details

Resolution: 25.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: Dynamo
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)