EMDB EMD-4939

Single particle reconstruction
3.3Å resolution

Structure of PTCH1 bound to a modified Hedgehog ligand ShhN-C24II: focused refinement of the membrane domain

Map released:
Overview of EMD-4939
Source organism: Homo sapiens [9606]
Related EM entry by publication: EMD-4936
Primary publication:
Structural basis of sterol recognition by human hedgehog receptor PTCH1.
Qi C, Di Minin G, Vercellino I, Wutz A, Korkhov VM
Sci Adv 5 eaaw6490-eaaw6490 (2019)
PMID: 31555730

Function and Biology Details

Sample name: Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II
Proteins: Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II, Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II, Complex of PTCH1 with a modified Hedgehog ligand ShhN-C24II, Protein patched homolog 1, Sonic hedgehog protein

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)