Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-49165

Single-particle
3.28 Å
EMD-49165
3D View Gallery
Deposition: 10/02/2025
Map released: 24/12/2025
Last modified: 24/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-49165
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9n9c summary report (pdf.gz) Map and 9n9c FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the dCas12f-gRNA complex

EMD-49165

Single-particle
3.28 Å
EMD-49165
3D View Gallery
Deposition: 10/02/2025
Map released: 24/12/2025
Last modified: 24/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Flagellimonas taeanensis
Sample: dCas12f-gRNA complex
Fitted models: 9n9c

Deposition Authors: Chang L , Sternberg SH, Xiao R , Hoffmann FT, Wiegand T, Xie D

Structural basis of RNA-guided transcription via Cas12f-Sigma factor association
Renjian X, Leifu C
To Be Published