EMDB EMD-4913

Single particle reconstruction
6.6Å resolution

CryoEM structure of the complete E. coli DNA Gyrase complex bound to a 130 bp DNA duplex

Map released:
Last modified:
Overview of EMD-4913
Source organisms:
Fitted atomic model: 6rkw
Related EM entries by publication: EMD-4909, EMD-4910, EMD-4912, EMD-4914, EMD-4915
Primary publication:
Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.
Vanden Broeck A, Lotz C, Ortiz J, Lamour V
Nat Commun 10 4935-4935 (2019)
PMID: 31666516

Function and Biology Details

Sample name: The complete E. coli DNA Gyrase complex bound to 130bp DNA duplex
Ligands: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, (3~{R})-3-[[4-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)piperidin-1-yl]methyl]-1,4,7-triazatricyclo[6.3.1.0^{4,12}]dodeca-6,8(12),9-triene-5,11-dione
Nucleic acids: DNA (58-MER), DNA (62-MER)
Proteins: The complete E. coli DNA Gyrase complex bound to 130bp DNA duplex, DNA gyrase subunit A, DNA gyrase subunit B, DNA, DNA gyrase subunit A, DNA gyrase subunit B

Experimental Information Details

Resolution: 6.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)