EMDB EMD-4912

Single particle reconstruction
4.6Å resolution

E. coli DNA Gyrase - DNA binding and cleavage domain in State 2

Map released:
Last modified:
Overview of EMD-4912
Source organism: Escherichia coli (strain K12) [83333]
Fitted atomic model: 6rkv
Related EM entries by publication: EMD-4909, EMD-4910, EMD-4913, EMD-4914, EMD-4915
Primary publication:
Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.
Vanden Broeck A, Lotz C, Ortiz J, Lamour V
Nat Commun 10 4935-4935 (2019)
PMID: 31666516

Function and Biology Details

Sample name: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 2
Nucleic acids: DNA Strand 1, DNA Strand 2
Proteins: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 2, DNA Gyrase, DNA, DNA gyrase subunit A, DNA gyrase subunit B

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)